Introduction to Digital PC
Digital PCR (dPCR) enables precise, highly sensitive quantification of nucleic acids. Traditional PCR is an end-point analysis that is semi-quantitative because the amplified product is detected by agarose gel electrophoresis. Real-time PCR (or qPCR) uses fluorescence-based detection to allow the measurement of accumulated amplified product as the reaction progresses. qPCR requires normalization to controls (either to a reference or to a standard curve), allowing only relative quantification. Furthermore, variations in amplification efficiency may affect qPCR results.
Digital PCR builds on traditional PCR amplification and fluorescent-probe–based detection methods to provide highly sensitive absolute quantification of nucleic acids without the need for standard curves. In the Droplet Digital™ PCR System, a PCR sample is partitioned into 20,000 droplets. After amplification, droplets containing target sequence are detected by fluorescence and scored as positive, and droplets without fluorescence are scored as negative. Poisson statistical analysis of the numbers of positive and negative droplets yields absolute quantitation of the target sequence.
Shipping & Delivery
All estimated shipping times are in addition to fulfillment times, We offer a next working day delivery for orders placed before 6:30 p.m. Monday to Friday. Orders placed after this will be delivered within two working days. This excludes Saturday, Sunday and holidays. Appointed is not responsible for any customs/duties related to international orders. We are unable to calculate charges prior to your order being delivered, and recommend checking with your local customs office for more information. Shipping fees will not be refunded if you refuse these charges.
- Free destination delivery above $100
- Europe 1 – 3 days Free
- United States 4 – 6 days Free
- Asia 3 – 6 days Free
- Africa 5 – 7 days Free
- Australia 3 – 5 days Free
Returns & Refunds
We have a 14-day return policy, which means you have 14 days after receiving your item to request a return, To be eligible for a return, your item must be in the same condition that you received it, unused, and in its original packaging. You’ll also need the order confirmation, order number, or proof of purchase. We will notify you once we’ve received and inspected your return, and let you know if the refund was approved or not. If approved, you’ll be automatically refunded on your original payment method. Please remember it can take some time for your bank or credit card company to process and post the refund too.
1 review for ddPCR System
There are no reviews yet.